An Enhanced Pre-Processing Technique for Evaluation of Cells, in vitro through Image Analysis

This paper proposes a method for denoising and enhancement in vitro images using various filters. It deals with enhancing the contrast of suspicious areas in the image and also involves the removal of background noise from the image. Lipoproteins (LDL+VLDL) were isolated, oxidized in vitro and used to differentiate monocyte into macrophage. Images were captured at the end of experimental period for image analyzing. This proposed technique can extract cells, in vitro information which is not apparent by visual inspection. The objective of preprocessing is to improve the quality of the image and make it ready for further processing by removing the irrelevant noise and unwanted parts in the background of cells, in vitro. The proposed method enhances the image into maximum level of transformation and produces good SNR ratio with minimum mean square error. Evaluate the cells; in vitro before proceed to the gene expression studies.