Detection of Cymbidium Mosaic Virus (CYMV) on Vanda Plants

Orchid plants are the members of Orchidaceae consisting of more than 25, 000 species, which are distributed almost all over the world but more abundantly in the tropics. There are 177 genera, 1, 125 species of orchids that originated in Thailand. Orchid plant collected from different nurseries showing Chlorotic and mosaic symptoms were observed on Vanda plants and it was suspected to infect with virus. So the symptomatic plants were tested for Cymbidium Mosaic Virus (CYMV), Odontoglossum ring spot virus (ORSV), Poty virus and Tomato Spotted Wilt Virus (TSWV) with Direct Antigen Coating- Enzyme Linked Immunosorbent Assay (DAC-ELISA) and further confirmed by Transmission Electron Microscopy (TEM). With the two methods CYMV and ORSV were detected positively from the suspected imported samples and low positive results were observed for Potex, Poty virus and Tomato Spotted Wilt Virus (TSWV).