Antimicrobial Efficiency of NaOCL and Helbo Laser against Enterococcus faecalis

The aim was to compare the efficacy of antimicrobial therapy, applying photodynamic therapy in different time intervals and the conventional method of irrigation of channel with sodium hypochlorite NaOCl 2.5 %. Material and methods. In our study were used 32 extracted teeth with single root. For inoculation are used types of bacteria Enterococcus faecalis ATCC 29212. In the first group of teeth (n=7) for the channel disinfection was used 2.5 % NaOCl, while in the second group (n = 21) were divided 7 channels (n = 7) were treated with Helbo laser for 1 minute, 7 channels (n = 7) of the second group were treated with Helbo laser for 3 minutes and other 7 channels were treated with Helbo laser for 5 minutes. In liquid cytometer at the same time controls are performed over 4 untreated teeth. Results. For statistical analysis of the results is used ANOVA with post- test Hokc, the program IBM SPSS Statistics 20. In our study at the group of channels treated with 2.5 % NaOCl, the number of dead cells after irrigation of the channel is 92, 55 (p=0.087 %). At the PDT group with the application of laser in the duration of 5 minutes the number of dead cells is 80, 06 % ( p = 0.003). With the application of laser in the duration of 3 minutes the number of dead cells is 83, 53 % ( p = 0.009). With application of laser in 1 minute durationthe number of dead cells for E. faecalis is 72.10 % (p less than 0.001). Conclusion. Irrigation with 2.5 % NaOCl should always be used for the treatment of chronic infections of tooth root canals with E. faecalis. Photodynamic therapy diode laser with X = 665 nm with a duration of 5 minutes has been relatively efficient