Phytochemical Content of Leaf and In Vitro Established Callus Culture of Pisonia alba Span

Callus culture of Pisonia alba span. were established from leaf explant. Different growth regulators greatly influenced the growth of callus cultures. Leaf derived callus grown on Murashige and Skooge (MS) medium fortified with different concentration (1, 1.5, 2, 2.5 ppm) of2, 4- Dichlorophenoxy acetic Acid, Indole - 3-acetic Acid, Indole - 3-butyric Acid Naphthalene Acetic Acid, 6- Benzyl Adenine or 6 Benzyl Amino Purine and Kinetin. Accumulation of bioactive compounds namely Diethyl Phthalate, 2-Pentadecanone, 6, 10, 14-trimethyl, n-Hexadecanoic acid, Hexadecanoic acid, ethyl ester, Phytol, 9, 12-Octadecadienoic acid, ethyl ester, Ethyl Oleate, Stigmasterol and gamma. -Sitosterol from callus culture was detected and confirmed by G C- Mass Spectrum Analysis.