Analysis of Monolaurin, Beta- Carotene, Weakened Beta-Carotene and their Antagonistic Properties with HIV: An Exploratory Study

The study is framed to find out the HDL variation, viral load obliteration, vulnerability in the oxidative stress with the introduction of pro-oxidants, HIV's RNA mutation in the selected HIV positive people or BLT mice with the combination of monolaurin and beta carotene, monolaurin and weakened beta carotene, in the presence of ART and Monolaurin and beta-carotene combinations, in the absence of ART and monolaurin and beta-carotene. The problems will be focused in the serological studies with the help of LC, HPLC, Cytometric techniques, Western Blot and genotyping test. The effect of the combination will be giving the status of CD4 counts in pre and posttest and it would be the major part to obtain the various results in the background of these combinations. The study is based on Exploration of missing links of monolaurin's viral properties and the limiting factor of monolaurin can work out with Beta carotene, either normal form or weakened form. Monolaurin sensitive to the LPS (Lipo Poly Saccharide) in the GST (Gastro intestinal Tract). That would be the base, to link up with the beta carotene, it need some support to penetrate to the LPS, this will be enhanced by the Beta carotene's properties either in the form of Antioxidant or Pro-oxidant. These combinations can create oxidative stress in the viral Nucleotides and make the RNA to mutate, so that that there will be no contact between host cells. To find out this study we will use the Western blot to compare the Protein sequences of normal HIV affected serum with the serum will have both combinations.