Prevention Urea Toxicity on Water Content of Muscles of a Freshwater Field Crab, Barytelphusa guerini by using an Additive, Methionine

The study of prevention of urea toxicity on a fresh water field crab, Barytelphusa guerini is most important aspect of this study. Fresh water field crab, B. Guerini collected from river Godavari at Nanded, and brought to the laboratory for acclimatization. After acclimatization they were exposed to various concentration of urea ranging from 5 to 40 g/L estimate LC50 value for 96 hr. After estimation of LC50 value (25 gm/L) they were exposed to sublethal concentration of urea (10 gm/L) to study the effect on Water content. It was observed that Water content was loss in urea treated crab, whereas Methionine treated crab shows increased in water content.