In- vitro Assessment of Non Starch Polysaccharide Hydrolyzing Enzymes in Broiler Diets Based on Guar Meal, Rape Seed Meal and Cotton Seed Meal

The present study was undertaken to assess the In-vitro sugar release with different non starch polysaccharide (NSP) hydrolyzing enzymes, viz. xylanase, -D- glucanase, cellulase, mannanase and pectinase in broiler diets based on corn +soya (Diet I) or supplemented with alternate protein (AP) sources like guar meal, rapeseed meal and cotton seed meal, each at two levels i. e.3 and 6 percent in diet (Diet II and Diet III, respectively). The NSP enzymes were initially supplemented individually to all the diets at different concentrations. Based on the data, the optimum concentration of each enzyme yielding maximum sugar was identified. The enzyme with maximum sugar release was further used in the enzyme combination studies at 3 different concentrations (i. e.100, 80 and 60 %) in each of the test diet. Similarly a combination of all the enzymes was made at the lowest concentration of each of the enzyme, which was tested at 100, 200 and 400 % on each of the test diet. The maximum sugar release observed in diet-I, II and III was @ 60, 100 and 60 % respectively. i. e. (xylanase, -D-glucanase, cellulase, mannanase and pectinase @ 2400, 4800, 1800, 4800, and 2400 IU/kg respectively for diet I, 4000, 8000, 1900, 10000 and 4000 IU/Kg respectively for diet II and 2400, 4000, 1800, 4800 and 2400 IU/kg respectively for the diet III). Similarly the lowest concentration studies of the enzymes with diet I, II and III revealed a significant sugar release @ 100, 200 and 200 % concentrations i. e. Xylanase -D-glucanase, cellulase, mannanase and pectinase @ 200, 120, 100, 100, and 200 IU/kg respectively for diet I, 400, 240, 200, 200 and 400 IU/Kg respectively for the diet II and diet III. It is concluded that the maximum sugar release was reported in the diet I @ 60 % of enzyme concentration.