Effect of Andrographis Paniculata to the Expression of IL-6, IL-17, IL-10, TGF?, and the Ratio of Treg / Th17 in Sprague Dawley Rats with Atherosclerosis Diet and Cigarette Smoke

Atherosclerosis is a chronic, active and progressive inflammatory disease of the arterial wall controlled by native and adaptive immune responses (Ren, et al 2010). Continuous exposure of oxidized LDL causes macrophages activated more and produces pro-inflammatory cytokines and proteases. The pro-inflammatory cytokines lead to the differentiation of naive T cells tend to become pro-inflammatory effector T cells such as Th1, Th2 and Th17 (T helper) than to counter-inflammatory Tregulator cells. The aim of this study was to determine the role of the ethanol extract of Andrographis paniculata in slowing the inflammatory reaction by increasing the ratio of Treg / Th17 cells and increasing the amount of expression of counter-inflammatory cytokines (IL-10 and TGF) than pro-inflammatory cytokines (IL-6 and IL-17) in Sprague Dawley rats with Atherosclerosis Diet and Smoke induction. This study uses a post-test experimental design control study. Samples taken at random. The research was conducted in vivo to demonstrate the effect of ethanol extract of Andrographis paniculata in a variety of dosage on the expression of IL-6, IL-17, IL-10, TGF- and the ratio of Treg / Th17 cells, in Sprague Dawley rats with Atherosclerosis Diet and Smoke induction The results showed that ethanol extract of Andrographis paniculata at a dose of 1.2 mg / kg in Sprague Dawley rats with Atherosclerosis Diet and Smoke induction, take effect on IL-17, and the ratio of Treg / Th17 cells (p less than0.05). Expression of IL-17 was significantly higher in the group of ethanol extract of Andrographis paniculata at a dose of 1.2 mg / kg compared to the negative control. Based on the above data, it can be concluded that the ethanol extract of Andrographis paniculata can reduce chronic inflammation and the risk of atherosclerosis in the blood vessels of Sprague Dawley rats with Atherosclerosis Diet and Smoke induction