Analyze the Expression of Cytokeratin 5 on the Epithelial Cells of the Buccal Mucosa in Batik Workers

The industry of batik is often use synthetic dyes as azo. Azo dyes contain naphtol and diazonium salts that are toxic to the tissue when exposed via inhalation, swallowing or direct contact. Some preliminary studies mentioned the exposure on the azo dyes batik could be expected to cause abnormalities in the buccal mucosal epithelial cell nuclei marked increase in the frequency of micronucleus, karyolisis, and pyknosis. Objective the purpose of this study was to analyze the expression of cytokeratin 5 on the epithelial cells of the buccal mucosa in batik workers Yogyakarta. Material and methods the study included 30 male subjects were divided into 2 groups 15 subjects exposed to azo dyes and 15 subjects as control. Criteria for subject were batik workers exposed azo dyes in The Unit of Coloring at batik home industry Yogyakarta, while control is not exposed to azo dyes. Buccal mucosal epithelial cells were swabbed using cytobrush. Analysis of the expression of cytokeratin 5 in the buccal mucosal epithelial cells was used monoclonal antibody cytokeratin 5 (Biocare Medical, USA) and immunohistochemical method (ABC Staining Kit, ImmunoCruz, Santa Cruz Biotechnology, USA). Data were analyzed using independent t-test. Results the results showed the expression cytokeratin 5 of epithelial cells buccal mucosa was significantly higher in batik workers group than the control (p less than0.05). Conclusion It is concluded that exposed azo could increase the expression of cytokeratin 5 in the epithelial cells of the buccal mucosa batik workers in Yogyakarta.