High Performance Liquid Chromatographic Method for Simultaneous Quantitation of Luteolin and Quercitin from Dried Whole Plant Powder of Gmelina Arborea Roxb.And Kalanchoe Pinnata (Lam.)Pers.

A simple reversed phase high performance liquid chromatographic (RP-HPLC) method was established for simultaneous quantification of luteolin and quercitin in the two medicinally important plants, Gmelina arborea Roxb. and Kalanchoe pinnata (Lam. ) Pers. Linear response was found in the concentration range of 1.0-500 g/mL for Gmelina arborea Roxb. and 0.5-500 g/mL for Kalanchoepinnata (Lam. ) Pers. respectively. The relative standard deviation for inter-day and intra-day precision was found to be less than2 %. Precision, specificity and robustness studies were carried out, values less than two ( % R. S. D) were observed. Recovery values from 98.53 to 98.93 indicated that the accuracy of the method was good. The proposed developed method was found to be precise, accurate and reproducible and can be used for routine quality control analysis and for the quantitation of quercitin and luteolin in herbal raw materials as well as in their formulations.