The Amelioration of Carbon Tetracholride ? Induced Toxicities by Methanolic Extract of Buchholzia coriacea Leave in Male Albino Rat

The study was designed to investigate potential ameliorative effect of methanolic leave extract of Buchholzia coriacea on carbon-tetrachloride (CCl4) - induced toxicities in male Albino rats. Thirty adult male Albino Rat were divided into five groups each comprising 6 animals, Group (I) the controls received only vehicles, olive oil (0.5 ml/kg bw) and DMSO, Group II (induction controls) received single dose of CCl4 (CCl4 + Olive oil in 11 ratio, 2 ml/kg bw, i. p, Group, IV and V received 200mg/kg bw, 400mg/kg methanolic extract of Buccholzia coriacea and 200mg/kg, Silymarin (50 mg/kg, o. p) as standard reference drug respectively after induction with a single dose of CCl4 for seven days. Evaluations were made for Lipid perioxidation and antioxidant activities, enzyme activities and some other biochemical parameters, haematology and pathology profile using standard methods. The methanolic leave extract of Buchholzia coriacea at 200mg/kg and 400mg/kg reduced the levels of AST and ALT, however, there was a significant (Pless than0.05) reduction of ALP when 200mg/kg and 400mg/kg dose of the leaves extract were administered. The treatment with 200mg/kg and 400mg/kg methanolic extract of Buccholzia coriacea was able to reduce the levels lipid peroxide with corresponding increase in activity of antioxidant GR, CAT, GPX, GSH compared with group treated with single dose of CCl4 only. The histopathology results revealed restoration of the normal or near normal histo-architecture and haematological toxicities were reversed at the doses of the plant extract tested. In conclusion, the inhibition of lipid peroxidation and concomitant increase of the endogenous antioxidant defence systems in serum and organ studied with subsequent restoration of the normal or near normal histo-architecture of the these organs aver or demonstrate the significant ameliorative and protective role of methanolic extract of Buccholzia coriacea at 200mg/kg and 400mg/kg.