Study on Differentiate Procedure of Alkaline Phosphatase Test by Conventional Colorimetric Method and Optimized Standard Method in Pasteurized Milk

In order to determine pasteurized milk, one of the enzymes milk phosphates, is measured. A negative phosphates result indicates that any pathogenic bacteria have been destroyed during pasteurization. If it is positive, it means the pasteurization process was inadequate and the milk may not be safe for human consumption and will have a short shelf life. In conventional method the milk samples are diluted with buffer at pH 10.2 and incubated at the temperature at 37C for 2hrs. Any alkaline phosphates present in the milk samples will liberate Para nitro phenol from artificially added disodium p-nitrophenyle phosphate which can be compared with the standard color disc. There are differences for measuring the phosphatase activity in between conventional method and Optimized Standard Method. So Chowdhury AP1 et. al. , applied Optimized Standard Method according to the recommendation of the German clinical chemistry association (Deutsche Gesellschaft fur Klinische Chemie, DGKC) at the purpose of showing its better performance than conventional procedure with in short time.