In vitro Plant Regeneration from the Nodal Explants of Spermacoce articularis L. f.

An in vitro regeneration of plantlets from the nodal explants of Spermacoce articularis L. f. , a member of Rubiaceae, has been conducted. Nodal regions with axillary buds were cultured on MS medium supplemented with different concentrations of BAP and KIN ranging from 5 to 25 M alone or in combination of both. Among the two cytokinins tested 10 M BAP was found to be the best for shoot proliferation. However, combination of KIN with 10 M BAP further enhanced the rate of shoot proliferation. Maximum number of 31.8 shoots per node with 100 % shoot induction frequency were observed on MS medium containing 10 M BAP and 4 M KIN. The regenerated shoots were cultured on half strength MS medium containing different concentration of IAA, IBA and NAA for root induction. Among these three auxins, 4 M IBA was found to be the best hormone for rooting. The well rooted plantlets were successfully hardened with 80 % survival rate.