Study of Mutagenic Efficiency of EMS and SA in Winged Bean (Psophocarpus tetragonolobus (L.) DC.)

The seeds of two varieties namely II-EC-178313 and 2I-EC-38825 of winged bean (Psophocarpus tetragonolobus (L. ) DC. ) were treated with Ethyl methane sulfonate (EMS) and Sodium azide (SA). The effectiveness and efficiency of mutagen is determined by observing frequency of mutations induced by mutagenic treatment. In the present investigation from results it is observed that effectiveness is reduced when concentration of mutagen is increased. The mutagenic efficiency increased with increase in concentration of mutagen. The mutation rates were calculated taking into consideration the mean values of efficiency for each treatment. This gives an idea about the average rate of mutation induction per mutagen. Taking into consideration of lethality and sterility, variations in mutation rates were recorded.