Callus Formation in Certain Mulberry Varieties of M5, V1, S36 and Anantha

Callus formation is central to many investigation and applied tissue culture procedures. Callus can be multiplied and later used to clone numerous whole plants. Since extensive callus formation can be induced by elevated hormone levels, tissue culture media designed to produce callus containg pharmacological additions of cytokinins and auxins. Callus tissue was green and friable on 2, 4-D medium, whereas on NAA and IAA medium brownish, compact callus was reported. Though the nature of callus in our present study was smooth and yellow in M5, V1, S36, Anantha, nodular and yellow colour was observed in M5 in the beginning, it has become compact and greenish patches appeared when sub-cultured on to the medium fortified with kinetin and IAA.