Effect of subacute malathion application on oxidative stress biomarkers

In this study, it was aimed to investigate the effects of malathion on oxidative stress biomarkers and liver enzymes and the protection of caffeic acid phenethyl ester against malathione. In the study, a total of 40 (200-240 g) adult male Spraque Dawley rats with 10 animals in each group were used. In the study, 5 ml / kg corn oil by gavage in the control (C) group, 40 mg / kg malathion by gavage in the malathion (MAL) group, CAPE (10 µmol / kg) intraperitoneally in the malathion + caffeic acid phenethyl ester (MAL + CAPE) group. After 1 hour, malathion (40 mg / kg) was administered by gavage, and CAPE (10 µmol / kg) was administered intraperitoneally to the caffeic acid phenethyl ester (CAPE) group. At the end of 15 days of application, the blood of the rats was intracardiac under ketamine / xylazine anesthesia and the animals were applied cervical dislocation method. Paraoxonase (PON), aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities and high density lipoprotein (HDL), malondialdehyde (MDA) and nitric oxide (NO) levels were analyzed in plasma obtained from blood samples. When the analysis results were evaluated, plasma PON activity and HDL levels decreased (P<0.01) in MAL and MAL+CAPE groups compared to the control group, while AST-ALT activities (P<0.05), MDA and NO levels increased (p<0.01) was detected. According to the results obtained from the study, it was determined that malathion caused significant changes in oxidative stress biomarkers, while CAPE could prevent these changes and be protective against malathion toxicity.