Antibacterial Activity of Papaya Leaves (Carica Papaya L.) and Guava Leaves (Psidium Guajava L.) Extract Combination towards Escherichia Coli and Staphylococcus Aureus

Papaya leaves (Carica papaya L.) were traditionally used by society in treating skin diseases such as acne and diarrhea. Papaya leaves (PsidiumguajavaL.) contained active compounds such as carotain alkaloids, antraquinone, saponins, steroids, tannins, and triterpenoids. Guava leaves had the ability to cure acute and chronic diarrhea. Guava leaves contained secondary metabolites such as flavonoids, alkaloids, polyphenols, and tannins.This study aimed to determine the antibacterial activity of a combination of papaya leaves and guava leaves extract against Escherichia coli and Staphylococcus aureus. The concentration of the extract combination used was 20%, 15%, 10%, and 5% with the antibiotic ciprofloxacin as a positive control and aquadest as a negative control. The results showed that the combination of papaya leaves and guava leaves extract (1:3) was effective in killing bacteria Escherichia coli and Staphylococcus aureus at a concentration of 20% with an average diameter of 12.50 mm and 12.41 mm. ANOVA test result with confidence level of 95% obtained p value = 0,000 (p<0,05) to conclude that there was a significant influence between the variation of combination and concentration of papaya leaves and guava leaves extract against Escherichia coli bacteria and Staphylococcus aureus. This study concluded that combination of papaya leaves and guava leaves extract (1:3) with concentration of 20% had an antibacterial activity against Escherichia coli and Staphylococcus aureus.