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Rapid, Reliable and Robust Approach for Extraction-Free RT-PCR based Detection of SARS-CoV-2in Clinical Setting to Expedite Large Scale Screening

Journal: International Journal of Science and Research (IJSR) (Vol.10, No. 11)

Publication Date:

Authors : ; ; ;

Page : 1378-1385

Keywords : SARS-CoV-2; COVID-19; RT-PCR; heat treatment; nasopharyngeal swab samples; Extraction free method; Pooling;

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Rapid, reliable and robust method for the detection of SARS-CoV-2 is an indispensable need for diagnostics. The development of diagnostic method will aid to address further waves of the pandemic potentially with rapid surveillance of disease; and to allay the fears. To meet this challenge, we have developed a rapid RT-qPCR method for the detection of 3 target genes or confirmatory genes in less than 30 minutes. The assay showed 100% sensitivity and 100% specificity when tested on 120 samples. We compared a conventional extraction based method with extraction-free method, and then further reduced the run time of extraction free method. Additionally, we have validated our rapid RT-qPCR method for the assessment of pooled sample. We here by propose a most reliable approach for the mass screening of samples with ease of operation at low cost. Finally we designed a single tube analysis method which provides qualitative as well as quantitative results in minimum time.

Last modified: 2022-02-15 18:49:35