Nested Polymerase Chain Reaction for Identification of Mitochondrial Cytochrome b Gene of Gazella Dorcas | BiomedgridJournal: American Journal of Biomedical Science & Research (Vol.7, No. 1)
Publication Date: 2020-01-17
Authors : Reem Rabie Mohammed Salih; Abd elrahim E Karrar; Imad Eldain E T Aradaib;
Page : 16-18
Keywords : Nested PCR; Blood; Dorcas; Gazell;
In this study nested PCR, was developed for rapid detection of mitochondrial cytochrome b gene. Whole blood in EDTA were collected from 17 Gazella dorcas from Hilat Kuku Zoo, Elkadaru's farm and Mozamel Elkurdi's farm in East of the Nile, Khartoum State. The origins of these animals are Dongola, River Nile- Northern Sudan and Butana- Central Sudan. The product was sent for sequencing to Macrogen Company- World Meridian 10F, Gasan-dong, Geumcheon-gu, Seoul, 153-781 Koreas. Nested PCR used DNA extracted from blood samples from Gazella dorcas by using GZ3 and GZ4 primers. The objective using this technique that nested- PCR is sensitive to detect the part of mitochondrial cytochrome- b gene of Gazella dorcas only. The primers were serially diluted (10 to 10-6) for sensitivity reaction. In all dilutions, the line was clear. The size of the product was 197 bp.
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