Agro Waste Mediated Production of Fibrinolytic Enzyme and Its Optimization and Application

Cardiovascular diseases (CVDs) are the leading cause of death globally. At least three-quarters of the world's deaths from CVDs occur in low-and middle-income countries. People living in low-and middle-income countries often do not have the benefit of primary health care programmes for early detection and treatment of people with risk factors for CVDs. Microbial fibrinolytic enzymes are very much considered as novel therapeutic candidate for the treatment of CVDs. The aims of this study were to screened and characterized Bacillus spp. isolate that could produce a natural Fibrinolytic enzyme with high activity. To develop safe and cheap thrombolytic agents, a fibrinolytic enzyme agro waste was used. BR1 and DGS1 was found to be potential strains for the production of enzyme production. After optimization potato peel showed better result by exhibiting maximum enzyme production i. e.2513 ? 49.2 U/g through SSF. The fibrinolytic enzyme were stable at pH 8 when incubated at 40?. In vitro clot lysis showed digestion of on goat blood clot. The method has proven to be highly simple and can be implemented to other enzymes also.