In-Vitro Evaluation of Antimicrobial & Antiulcer Activity of Aqueous Extract of Psidium Guajava

Aim and objective: The aim of the present study was to develop the In- Vitro Evaluation of Antimicrobial & Antiulcer Activity of Aqueous Extract of Psidium Guajava. It is defined as disruption of the mucosal integrity of the stomach and/or duodenum leading to a local defect or excavation due to active inflammation. Material and method: Psidium guajava or commonly known as Guava is a popular fruit tree. It is evergreen, tropical, and small growing only up to 10 m high. Leaves of Psidium guajava were washed with dechlorinated water, dried in shade and powdered with the help of an electric blender. The test materials (1.0 kg) were extracted with different organic solvents viz., acetone, hexane, petroleum ether, chloroform and methanol in a Soxhlet apparatus for 8 h and the extract was concentrated in a rotary vacuum evaporator to yield crude extract, which was used in bioassays. Result & discussion: H+/K+ - ATPase Inhibition Activity: The H+/ K+ - ATPase inhibition activity of aqueous extract at a various concentration (20?g, 40?g, 60?g, 80?g, 100 ?g) has compared with Omeprazole as standard. The extract significantly showed activity in a dose-dependent manner. Maximum percentage inhibition of 62.18?0.54% has been observed for extract at a concentration of 100?g, and standard Omeprazole showed 69.56?1.72%.