Review on Current Trends of Gas Chromatography in Pharmaceutical Industry

A gas chromatograph (GC) is an analytical tool for determining the composition of distinct segments in a sample. GC may help in recognizing a compound. In preparative chromatography, GC can be used to get ready pure compound from a mixer. A gas chromatograph is a chemical analysis tool that is used to separate compounds from a complex sample mixture. In GC the mobile phase is inert gas and stationary phase is a thin layer of inert fluid on an inert solid support. It has high resolution power compared to other methods. The GC method is highly accurate quantitative analysis typical of 1-5%. It is limited to volatile sample. It requires spectroscopy, usually mass spectroscopy, for conformation of peak identity. The Instrumentation involves sample injection-A sample port is necessary for introducing the sample at the head of the column. Carrier Gas-The carrier gas plays an important role, and varies in the GC used. Helium is most commonly used as carrier gas because it is safer than other gases. Autosamplers-Autosamplers are instruments that automatically take a sample and insert it into the GC for analysis. Columns-columns plays key role in GC there are two main types of columns are Open tubular columns and Packed columns. Detectors-A detector is a device used to detect components of mixture being eluted off the chromatographic column. Two main types of detectors mainly used in GC are Flame Ionization Detector (FID) Thermal Conductivity Detector (TCD).