CHLORBENZENES, ORGANOCHLORINATED PESTICIDES AND PCB IN BIOTA SAMPLES OF KARAVASTA LAGOON

In this paper are presented concentrations of chlorobenzenes, organochlorinated pesticides and polychlorinated biphenyls (PCB) in biota samples of Karavasta Lagoons. Fish and mussel samples were taken in September 2014. Ultrasonic extraction was used for extracting organochlorinated pollutants from tissues of biota samples. Clean-up procedure were realized using firstly silicagel treated with 45% sulphuric acid for lipid hydrolysis followed by a second clean-up procedure in an “open” florisil column. Analysis was realized in HP 6890 Series II, gas chromatograph equipped with μECD detector. For separation of chlorobenzenes, organochlorinated pesticides and PCB markers was used Rtx-5 capillary column (30m x 0.32mm x 0.25μm). Mono to hexachloro benzene was determined in fish samples. The organochlorine pesticides detected were: HCHs (a-, b-, γ- and d-isomers) and the DDT-related chemicals (o,p-DDE, p,p-DDE, p,p-DDD, p,p-DDT), Heptachlors, Aldrines and Mirex. Analyzes of PCBs was based on the determination of the seven PCB markers. Organochlorinated pollutants were found for all analyzed samples. Monochlorobenzene was found higher than other chlorinated benzenes. The main origin of organochlorine pesticides could be as result of their previous uses in agricultural areas near the lagoon. PCB 28 and PCB 118 were found in higher level than other congeners. These levels were comparable to levels reported from previous studies for this ecosystem. Cluster analyze was used for interpretation of data found for biota samples of Karavasta Lagoon.