Phylogenetic Cut-off Values for Species and Genera Discrimination of Yeast

In the article, we give a brief presentation of our new method based on a unified fram Lodderomyces and Kazachstania clades. Furthermore, a new approach has been applied to discriminate yeast species and genera in the Starmerella clade. An indication of this is numerous literature data showing low statistical support for basal branches of a phylogenetic tree of the Starmerella clade. Phylogenetic analysis shows that a tree of the Stramerella clade falls into 7 clusters with high statistical support of basal branches. Among these clusters, cluster 5 represents a new genus. At the border of cluster 5 and its neighbor cluster 4, the fol-lowing characteristics change with a jump: nucleotide sequence identity (SI), evolutionary distance (ED), and physiological profiles (PP) between species belonging to clusters 4 and 5.