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Investigation of the Effects of Medium and FBS on Cell Proliferation in Different Cell Lines

Journal: Süleyman Demirel University Faculty of Arts and Science Journal of Science (Vol.17, No. 1)

Publication Date:

Authors : ; ; ;

Page : 55-64

Keywords : Cell Culture; Medium; FBS; MTT; Proliferation;

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Cell culture is an artificial, sterile environment created to maintain the viability and proliferation of cells under laboratory conditions. In these artificial environment conditions, in addition to the medium, cell proliferation enhancing substances such as Fetal bovine serum (FBS) are added to support the growth of cells. Cells can proliferate more effectively if appropriate medium and FBS ratio are used. In this study, it was determined that different media types (Dulbecco's Modified Eagle's Medium (DMEM), RPMI-1640, DMEM High-Glucose and DMEM/F12) and different FBS ratios (0%, 5%, 10%, 15%, 20%, 25%) effects on cell proliferation in determined cell lines (L929, J774, Arpe-19, RVECs, AU-565, MCF-7, HEK293T) were examined. Cell viability analyzes were performed with the MTT test. According to the results of the MTT test, the most suitable medium type on which the cell lines will proliferate and the FBS ratios to be used were determined. With this study, it was determined that the specified cell lines could proliferate in many media types and at different FBS ratios. When cell studies are carried out considering the study data, the production of cells will be carried out using the most effective medium type for different cell lines and the FBS ratio at which optimum cell proliferation occurs. In this way, it is aimed to produce cells by using FBS, which is one of the most costly products in cell studies, at the most ideal level.

Last modified: 2022-12-08 18:06:46