Detection of Canine Parvovirus in Dogs by Means Polymerase Chain Reaction |Biomedgrid

Worldwide, the infection with canine parvovirus type 2 (CPV-2) is one of the main causes of hemorrhagic enteritis in dogs and having a diagnostic technique that is highly sensitive is essential for veterinarians, dog owners and breeders. In this work, a protocol was implemented that uses the conventional Polymerase Chain Reaction (PCR) to detect a CPV-2 DNA fragment from feces of dogs with clinical signology corresponding to canine parvovirus. In total, 12 stool samples that were positive with conventional PCR were collected and analyzed, which was confirmed by sequencing the fragments obtained and contrasted with the sequences of the different variants of CPV-2 described in the GenBank database.