Antimicrobial potential of thiophene Derivatives

New series of 2,5-disubstituted thiophenes were synthesized. Thiosemicarbazones 1a-b were reacted with various reagents, such as diethyl-2-bromomalonate, ethyl-2-chloroacetoacetate, thioglycolic acid, 4-4-substituted phenacyl bromides, and acetic anhydride, to afford heterocyclic substituted thiophene derivatives a-b, 3a-b, 4a-b, 5a-b, 6a-b, and 7a-b, respectively. Moreover, cyclization of the key intermediate b with chloroacetic acid yielded thiazolidine 8, which on reaction with appropriate aromatic aldehydes afforded the corresponding arylidene derivatives 9a-f. Finally, reaction of N-arylidene cyanoacetohydrazide 10 with sulfur and phenylisothiocyanate yielded thiazoline derivative 11, which on treatment with triethylorthoformate and acetic anhydride afforded thiazolo[4,5-d]pyrimidinone derivative 12. Some of the newly synthesized compounds showed promising anti-inflammatory activity