DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTIFICATION OF ACYCLOVIR IN TABLETS

A reverse phase method has been developed for the quantitative estimation of Acyclovir in tablet. The quantification was carried out using RP stainless steel column Water HEMA C18 (250 x 4.6) mm 5μ column packing in isocratic mode with mobile phase containing methanol, acetonitrile and water in the ratio of 45:45:10, Flow rate of 1.0 ml/min and the detection wavelength were set at 264 nm and the linearity was found to be in the range of 2-10 ?g/ml for acyclovir. The proposed method was found to be simple, precise, accurate, and reproducible for the estimation of acyclovir. Keywords: Acyclovir, method development, validation, high performance liquid chromatography.