Isolasi dan Identifikasi Bakteri dari Tinja Orangutan Penderita Gangguan Gastrointestinal (BACTERIAL ISOLATION AND IDENTIFICATION IN FAECES OF ORANGUTAN WITH GASTROINTESTINAL DISTURBANCE)

Orangutans are among protected animals by the law. One of orangutans’ main health problems is gastrointestinal disease due to bacterial infection. Microbiological data of causative agent of illness in orangutan still not much reported scientifically. This research aim was to identify causative agent of bacterial infection on gastrointestinal disorder in orangutan isolated from stool samples. The samples were collected from Yayasan Konservasi Alam Yogyakarta and Borneo Orangutan Survival, Semboja, Kalimantan Timur. Fresh fecal samples were collected using sterile swab and put them into a sterile transport media. To achieve pure cultures, bacterial isolation was performed by using plate streaking on selective media. Gram stain was done to confirm the cell uniformity and morphology. Bacterial identification was performed according to Bergey’s Manual Determinative Bacteriology on some biochemical characters to determine the isolated bacteria. The result showed that three bacteria were identified from stool samples orangutan from Yayasan Konservasi Alam Yogyakarta, i.e.: Citrobacter amalonaticus, Providensia rustigianii, and Proteus mirabilis. Meanwhile, three bacteria, which were Klebsiella planticola, Enterobanter agglomerans and Escherichia coli, were also identified in samples taken from Borneo orangutan.