IN VITRO ANTIBACTERIAL ACTIVITY OF CRUDE METHANOLIC EXTRACTS FROM LEAVES OF JASMINUM SAMBAC

This study was carried out with an objective to investigate the antibacterial potentials of leaves of Jasminum sambac. In the present study, the antibacterial activity of methanolic extracts of leaves of Jasminum sambac was evaluated for potential antibacterial activity against medically important bacterial strains. The antibacterial activity was determined in the extracts using agar disc diffusion method. The antibacterial activity of extracts (25, 50, 100,250,500μg/ml) of Jasminum sambac were tested against four Gram-positive bacteria?Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Sarcina luteae and four Gram-negative bacteria?Escherichia coli, Pseudomonas aeruginosa, Salmonella paratyphi, Shigella dysenteriae which are human pathogenic bacteria. Zone of inhibition of extracts were compared with standard Kanamycin disc at a dose of 25μg/disc for antibacterial activity. Significant antibacterial activity was found at a dose of 500μg/ml of methanolic extract. It was found 17 mm, 14 mm, 15 mm and 13 mm zone of inhibition against Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Sarcina luteae respectively and 14 mm, 15 mm, 15 mm and 16mm zone of inhibition against Escherichia coli, Pseudomonas aeruginosa, Salmonella paratyphi, Shigella dysenteriae respectively. The results showed that the methanolic extracts of Jasminum sambac have the significant inhibition of the bacterial growth against the tested organisms. The phytochemical analysis of the plant was carried out. The presence of alkaloids, tannins and resins are revealed in phytochemical analysis. Key Words: Jasminum sambac, Antibacterial activity, Diffusion Method, Kanamycin, Zone of inhibition