Clinical and histopathology evaluation of cutaneous Pythiosis in calves the department of Cordoba, Colombia

Objective. Evaluate and characterize the clinical and histopathological aspects of cutaneous Pythiosis in calves the department of Córdoba, Colombia. Materials and methods. A descriptive, non?probabilistic, convenience study was conducted in animals. 14 calves were with skin Pythiosis were analyzed, which underwent clinical and histopathologic characterization using hematoxylin?eosin and Grocott staining. Results. Clinically, the animals developed humid skin ulcers with abundant fibrin bloody exudation, some had irregular surface nodules and bleeding and in some cases, yellowish white caseous material. Lesions were located in most cases, on the distal parts of the limbs and the ventro?lateral region of the neck. Histopathologically, in hematoxylin?eosin staining, pyogranulomas and a multiple central area constituted by agglomerates of eosinophils and neutrophils, some of them surrounded by macrophages and giant cells (Splendore?Hoeppli reaction) were observed. The Grocott stain showed multiple septate and partially branched, intralesional, straight, brown hyphae. Conclusions. The definitive diagnosis of the disease was based on epidemiology, macroscopic characteristics of lesions and histopathologic findings, as being conclusive diagnostic methods for bovine cutaneous Pythiosis.