HEMATOLOGIC AND MOLECULAR GENETIC STUDIES OF ANIMAL BLOOD FOR TESTING PREPARATION "LEYKOZAV" AGAINST THE VIRUS BOVINE LEUKEMIAJournal: Scientific reports of NULES of Ukraine (Vol.4, No. 61)
Publication Date: 2016-07-18
Authors : A. A. Zaviriukha; U. M. Yanenko; O. Y. Balaban;
Page : 1-14
Keywords : leukemia; diagnosis; hematology; ELISA; PCR; cattle;
This paper presents the results of the blood of cattle research in leukemia cattle. It was found that the formation of antibodies against leukemia virus in the serum of cows blood begins after 6 months in the animals in the herd unfavorable. Polymerase chain reaction in real time was a more sensitive diagnostic method than the reaction of immunodiffusion. By using PCR found that the beginning of the accumulation of proviral nucleic acid leukemia occurs in the sixth month of the animal's stay in a dysfunctional herd. Preventive immunization of cows suffering from leukemia experimental drug "Leykozav" reduces the amount of proviral DNA copies of leukemia in the blood to 7-420 times. The animals of the control group for the period of the experiment marked increase in the number of leukocytes by 34% compared to normal. 6 months after immunization preparation "Leykozav" the concentration of white blood cells in the experimental cows decreased, but are within the normal range. In order to maintain stability on the well-being of leukemia in herds of cattle is recommended to vaccinate all animals once a year, in spring or autumn, the preparation "Leykozav", observing the rules of asepsis and antisepsis.
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