Activation and comparative kinetics of Ovis aries plasmin with human plasmin

Objective. Compare the kinetics and activations, unify the purifications methods and establish the sequence of the N-terminals of the plasminogens human and Ovis aries. Materials and methods. The plasminogens were purified by the same methods: affinity and ion exchange chromatographies, furthermore both plasminogen were activated by human urokinase and the N-terminals sequence perform by the Edman’s degradation’s method. Results. The affinity of Ovis aries plasmin for the chromogenic substrate was determined in 0.45 mM, 11.8 times more affinity that the human plasmin (5.3 mM). Conclusions. The method of the plasminogens purification and the unification was established for all mammals. The high affinity of the Ovis aries plasmin confirmed a mayor affinity of animal’s plasmin to the chromogenic sustrate, compared with the human plasmin.