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Detection of Escherichia coli O157: H7 and Salmonella in pigs ofdepartment of Cordoba

Journal: REVISTA MVZ CÓRDOBA (Vol.9, No. 1)

Publication Date:

Authors : ; ; ;

Page : 386-392

Keywords : Escherichia coli O157:H7; Salmonella spp.; porcines; Córdoba; Colombia.;

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Abstract

E. coli O157: H7 and Salmonella spp are bacteria of world wide distribution that caused intestinal tract disease either humans and animals. The present study had ass objective to establish the frequency of E. Coli O157: H7 and Salmonella in the different systems of porcine production in the department of Cordoba. A prospective descriptive study by random samples in the porcine intensive and extensive systems was carried out. Five hundred faecal porcin samples, 250 belong to extensive system and 250 intensive systems were analyzed. The sample size was estimated on the population base of 111.672 porcines. For the detection of E. coli a pre-enrichment with peptone water 1% to 24h at 37 °C was undertaken, after a subculture on MacConkey sorbitol agar during 24h at 37°C was carried out. For salmonella spp a selective pre-enrichment Rappaport vassiliadis and tetrationate broths for 24h and subculture onto selective media were done. The results showed a frequency of salmonella spp of 1% in fecal porcines, 0.2% in the intensive system and 0.8% in the extensive system. Regarding the municipality distribution, the intensive system showed a frequency of Salmonella of 1.4% in Cienaga de Oro, 0% in Berastegui, El Quemao and Monteria. In the extensive system Salmonella was found in 3.75% in Cienaga de Oro, 0% in Berastegui and Monteria and 1.6% in El Quemao. No E. Coli O157:H7 was found. The antibiotic susceptibility test showed a sensitivity of 100% to trimethoprim-sulfomethoxazole, amikacin, ceftriaxone, ciprofloxacin, gentamicin, aztreonam and 20% of sensitivity to ampicillin & sulbactam were found. The work showed a low frequency of Salmonella spp in faecal samples of porcines and no presence of E. coli. However, it should be keeped the surveillance and epidemiological studies on these pathogens.

Last modified: 2016-10-28 23:24:04