Study on Liquid Chromatography and Mass Spectrometry of Memnoniella echinata Metabolites against Vector of Dengue and Chikungunya

Aedes aegypti and Ae.albopictus are vectors for transmitting Dengue, Chikungunya, and Yellow fever. These fevers are growing public health problem globally. Memnoniella echinata is a cosmopolitan fungus. This fungi is exceptionally good model for bio control and more significant as bio agent. Recently, especially fungi, has emerged as a current and future candidate for mosquito control. In the study M. echinata was cultured in the Potato Dextrose Broth (PDB) medium. The metabolites were filtered with the Whatman-1 filter paper. These purified metabolites were tested in the statistically significant concentrations of 50, 100, 150, 200, 250 ppm against first, second, third, and fourth instar larvae of Ae. aegypti and Ae. albopictus. The bioactive compounds have been isolated with the Thin Layer Chromatography (TLC) plates with chloroform and methanol used as solvent system for analysis. These compounds were detected with the Liquid Chromatography Mass Spectrometry (LC-MS/MS) analysis. The LC50, LC90 and LC99 values were 84, 188, 205 ppm against first and second instar. However, in case of third instar 115, 191, 209 ppm and 125, 196, 214 ppm was observed against fourth instarlarvae of Ae. aegypti. Thus, the LC50, LC90 and LC99 values were 84, 188, 205 ppm against first and second instar of A. albopictus. The third instar LC50, LC90 and LC99 values were 84, 192, 210 ppm, and fourth instar 89.2, 196, and 215 ppm respectively after exposure for 24 hours. These new results significantly support broadening the current dengue and Chikungunya vectors control paradigm beyond chemical larvicides.