Rapid Analysis of Moenomycin A Residue in Poultry Tissues Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry

A rapid, sensitive and accurate method has been developed for determination of Moenomycin A residue in poultry tissues. It was based on procedures with simple pretreatment process and Ultra High Performance Liquid Chromatography coupled to triple quadrupole tandem Mass Spectrometry (UHPLC-MS/MS). The procedures were deliberately optimized to tailor for complicated molecule and matrix. No significant matrix effects were observed after simple pretreatment and fast analysis within 10 min. Competent linearity across the concentration levels from 5 to 1000 μg.kg-1 was found with linear determination coefficient (r2) higher than 0.990. Limit of Detection (LOD) and Quantification (LOQ) were down to 0.25 μg.kg-1 and 0.8μg.kg-1 respectively. The mean recoveries from spiked blank kidney tissues at the levels of 10, 50, 500 μg.kg-1 ranged from 67.2 to 89.4%. Repeatability and within-laboratory reproducibility were lower than 7% and 11%. Finally, the proposed method was applied to real tissue samples and positive results were confirmed with the highest concentration up to 74.5 μg.kg-1.