Association between allele 12Pro of rs1801282 polymorphism gene PPAR and diabetes mellitus type 2

Aim ? to investigate the presence of polymorphisms and alleles in patients with type 2 diabetes mellitus (DM) and analyze the link of polymorphism Pro12Ala (rs1801282) in PPARG gene and incidence of diabetes type 2 in the Ukrainian population. Materials and methods. The study was conducted in 88 patients with DM type 2, the control group consisted of 50 patients. For molecular genetic research PCR in real time was used. For the analysis of polymorphic DNA loci standardized test system TaqMan Mutation Detection Assays Life-Technology (USA) was used. Results and discussion. In type 2 DM genotype Pro12Pro was found in 84% of patients versus 72% in the control group; genotype Pro12Ala - 16% vs. 24%; Ala12Ala genotype in patients was not recorded in any patient (in control - 4%). 12Pro allele was found in 92% of patients versus 84% in the control, allele 12Ala ? 8% vs. 16% in control. These differences were statistically significant for alleles (p(χ2)=0,04), but not for genotypes (p(χ2)=0,075). Any link between genotypes and DM type 2 was not recorded (p=0,07). Instead, such an association (p=0,04) was found for alleles: 12Pro (OR=2,20; CI=1,03-4,73) and 12Ala (OR=0,45; CI=0,21-0,97). Conclusions. In this study the probable association of gene polymorphism Pro12Ala (rs1801282) gene PPARG with the DM type 2 morbidity in the Ukrainian population was not found, but statistically significant association for allele 12Pro was evident, the presence of which increases more than 2 times the risk of disease, whereas the presence of the allele 12Ala reduced this risk.