DIFFERENTIAL DIAGNOSIS OF Entamoebaspp IN STOOL SAMPLES USING POLYMERASE CHAIN REACTION

Objective: Diagnosis of E. histolytica and E. dispar by conventional PCR and determine epidemiology for both parasites by conventional PCR Methods: (40) stool sample from suspected patients with E. histolytica /E. dispar and (50) sample of healthy individuals as a control, were examined by direct smear method and wet preparation method to differentiated for infection with E. histolytica and E. dispar by CP5 and ED-1 genes. Result: The results showed that (72.5%) were positive for CP5 gene for E. histolyticaand (27.5%) was negative for CP5 gene but positive for the specific primer (Ed-1) of E. dispar. Conclusion: Our finding suggests that CP5 gene could be using as a specific diagnosis gene for differentiation between E. histolytica and E. dispar.