The Biosynthesis of Antibiotics as a Taxonomic Marker of Some Species of the Genus PseudomonasJournal: Naukovi Visti NTUU KPI (Vol.22, No. 3)
Publication Date: 2017-06-23
Authors : Vitalii Klochko;
Page : 29-33
Keywords : Species of the genus Pseudomonas; Antibiotics; Taxonomy;
Background. The ability to biosynthesis of antibiotics was established for 20 of 250 species of Pseudomonas. The value of the biosynthesis of antibiotics as a taxonomic characteristic for the genus Pseudomonas is not studied. This issue will be analyzed using the experimental results. Оbjective. The aim of the paper is to identify the ability of usage of antibiotics synthesized by certain types of bacteria of the genus Pseudomonas, as a taxonomic marker. Methods. Methods of thin-layer chromatography (TLC) and LC/MS-analysis were used to determine the synthesis of antibiotics. Results. A unique set of antibiotics was determined for some types of bacteria Pseudomonas, which are specific for them only: pyocyanin – P. aeruginosa, 2,4-diacetylphloroglucinol – P. brassicacearum, 2-hydroxyphenazine-1-carboxylic acid – P. chlororaphis subsp. aureofaciens, pyrrolnitrin and 2,4-diacetylphloroglucinol – P. protegens, batumin – P. batumici. Conclusions. The ability to synthesize certain antibiotic substances may serve as a taxonomic marker and be used to identify certain species of Pseudomonas. However, the biosynthesis of antibiotics did not show specificity for several species. This problem requires a deeper study of different types of Pseudomonas.
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