Activity of hepatic antioxidant system under bisphenol administration and differential supplementation with retinoids

Bisphenol A (ВРА) is the potential hepatotoxin and widely used as a monomer in the production of ВРА-containing polycarbonate products. Its classic xenoestrogen toxic effects are well-described, the molecular mechanisms of bisphenol A-induced hepatotoxicity are not completely characterized and need to be further studied. It is known that introduction of ВРА is accompanied by development of oxidative stress. The work is aimed to assess enzyme activity of hepatic antioxidant system under administration of bisphenol A and differential supplementation with retinoids. The estimation of enzyme activity of the antioxidant system was conducted on the basis of determination of enzyme activity of catalase, superoxide dismutase and glutathione peroxidase in mitochondrial and cytosolic fractions of liver. It is demonstrated that administration of bisphenol A wild-type animals, with normal supplementation of vitamin A accompanied by the decline activity of catalase, superoxide dismutase and glutathione peroxidase only in cytosolic fractions of live. It is primarily related to the features of metabolism of bisphenol and localization of its biotransformation. However, the activity of these enzymes in the mitochondrial fraction unchanged and statistically not significantly different from that of the control group. In contrast, in animals deprived of endogenously-deposited retynil esters in the liver, superoxide dismutase, catalase and glutathione peroxidase both cytosolic and mitochondrial fractions of liver were level indicators in the control group who did not receive bisphenol A.