ESTIMATION OF TINIDAZOLE IN ALBINO RAT PLASMA BY USING RP-HPLC
Journal: Indian Drugs (Vol.54, No. 6)Publication Date: 2017-06-30
Authors : Debnath M.; Y. Indira Muzib; S. Ashutosh Kumar;
Page : 48-52
Keywords : Tinidazole; RP-HPLC; ICH guideline; Validation; Albino Rat Plasma; US-FDA guideline.;
Abstract
A new RP-HPLC method for the quantitative determination of tinidazole in albino rat plasma was developed and validated as per US-FDA guidelines. The drug was spiked in the albino rat plasma and extracted with mobile phase by precipitation method. The extracted analyte was injected into Hypersil ODS C 18 (4.6 x 150 mm; 5 μm) or equivalent, maintained at 25°C temperature and effluent monitored at 310 nm. The mobile phase consisted of potassium dihydrogen phosphate [pH 3.0]: acetonitrile [HPLC Grade] (40:60 v/v). The flow rate was maintained at 1.0 mL/min. The developed method shows high specificity for tinidazole. The calibration curve for tinidazole was linear from 1.0 to 40.0 ng/0.5mL plasma (r 2 = 1). The inter-day and intra-day precision was found to be within limits. The average % recovery for the drug tinidazole was found to be 99.53 - 100.21 % and the reproducibility was found to be satisfactory. The proposed method was adequate, sensitivity, reproducibility, and specificity for the determination of tinidazole in albino rat plasma.
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