Development of Data Acquisition Algorithm for Spectrometer for Fluorescence Applications

Abstract Emission and reflection of light from the objects depends on their internal energy levels and composition present in the environment. The hot objects emit light while cold objects reflect or absorb light on some specific wavelengths. Spectrometer is equipment which is used to measure the spectra of light emitted. This project aims to use Ocean Optics USB 4000 spectrometer to measure the intensity spectra of light incident on its input port. The control and data acquisition of spectrometer will be developed under EPICS (Experimental Physics and Industrial Control System). The signals acquired from spectrometer will be then decomposed into small functions by using EMD (Empirical Mode Decomposition). The conspired solution for removing noise from the signals which are nonstationary and non-linear in nature is done by using EMD. Available processing techniques are either linear and stationary or nonlinear and stationary so they are unsuitable for the random signals received from the sample object. The objective of the processing technique is to bring out the useful data from the signal received after reconstruction and accurately measuring the position with the help of time of flight. This spectrometer finds several applications in Agricultural Measurements and Monitoring, Polymer Analysis, Medical Diagnostics etc. Enabling it with EPICS allows it to easily communicate and control along with other device like laser, imaging system, time synchronization etc. involved in experiment.