ANTIDIABETIC PROPERTIES OF VARIOUS EXTRACTS OF HIPPOPHAE RHAMNOIDE

The various extracts of Hippophae rhamnoide plant have been scrutinized for the anti-diabetic property by the use of alpha-amylase and alpha glycosidase enzymes inhibition method. The various parts of the plant have been extracted by Soxhlet extraction method, in which various solvents have been used in the order of their increasing polarity like Petroleum ether, Ethanol and Water. Acarbose have been used as a reference antidiabetic medicine by the inhibition of alpha-amylase and alpha glycosidase enzyme, the two key enzymes responsible for the degradation of higher carbohydrates into lower glucose molecules. Three different plant parts have been analysed separately viz, root, leaves and fruit. The fruit extracts of the plant have been found to posse's highest inhibition potential followed by leaf extracts and least inhibition of alpha-amylase and alpha-glycosidase have been noticed for all root extracts. As per the solvent polarity, the highest inhibition of alpha-amylase and alpha glycosidase have been noticed for water extracts of the root, leaf and fruit extracts of the concerned plant, followed by ethanol extracts, and less inhibition of alpha-amylase and alpha glycosidase have been observed for petroleum ether extracts. Keywords: Hippophae rhamnoide, alpha-amylase, alpha glycosidase, antidiabetic, plant extracts