THE USE OF GLUCOSE AND DIMETHYL SULFOXIDE IN THE LOW-TEMPERATURE PRESERVATION CYANOBACTERIA OF SPIRULINA AND ARTHROSPIRA GENUS
Journal: Science and world (Vol.2, No. 57)Publication Date: 2018-05-29
Authors : Lykov I.N. Petrukhina D.I.;
Page : 14-18
Keywords : Spirulina subsalsa; Arthrospira platensis; cyanobacteriums; cryogenic storage; cryopreservation; glucose.;
Abstract
This work is devoted to the study of the possibility of cryopreservation of cyanobacteria at –80 °C in the presence of glucose as a cryoprotector. The article presents the results of comparative experiments on cyanobacteria culture cultivation before and after long-term cryopreservation. It is established that optimal cryoprotectants are 10 % solution of glucose and combination of 10 % glucose + 10 % dimethyl sulfoxide. The obtained data indicate that S. subsalsa and A. platensis retain good viability after 12 months of cryopreservation.
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