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Validated HPLC method for the estimation of Aspirin and Omeprazole in dosage form

Journal: Journal of Pharmacology and Biomedicine (Vol.2, No. 3)

Publication Date:

Authors : ;

Page : 171-180

Keywords : Aspirin; Omeprazole; HPLC; Chromatography; Spectrophotometry;

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Abstract

A new simple, rapid, selective, precise and accurate high-performance liquid chromatographic method has been developed for the estimation of aspirin and omeprazole in marketed formulation. The RPHPLC method was developed for estimation of aspirin and omeprazole in bulk and tablet dosage form by isocratically using 20 mM KH2PO4: acetonitrile (pH 3.5) in the ratio of 20:80 v/v as mobile phase, Thermo C-18 column (4.6 x 250 mm, 5μ particle size) column as stationary phase and chromatogram was recorded at 275 nm. Then developed method was validated by using various parameters. The parameters linearity, precision, accuracy, robustness, limit of detection and limit of quantitation were studied according to International Conference on Harmonization guidelines. The proposed method was found to be accurate, repeatability and consistent. It was successfully applied for the analysis of the drug in marketed formulation and could be effectively used for the routine analysis of formulation containing the drug without any alteration in the chromatography conditions.

Last modified: 2018-11-02 19:59:11