PATHOMORPHOLOGICAL INVESTIGA-TION OF SOFT FACIAL TISSUE IN THE APPLICATION OF NON SURGICAL LIGATURAL CORRECTION OF AGE SKIN CHANGES

Aim. To establish expediency of use of ligature correction by introduction of resorbing and permanent implants on the basis of study pathomorphological changes in a skin. Materials and methods. The studies were per-formed on 130 mature white male rats of the "Vistar" line weighing 100-130 g. Experimental animals were divided into 2 groups depending on the type of implanted threads. The rats of all groups (14, 30, 90, 180, 365 and 540 days) were divided into 3 subgroups each of them consisting from 10 individuals. For all 10 rats of each subgroup, the same type of suture material was implanted. The following Aptos threads were used during the experiment: Nano Spring (NS) and Light Lift Spring (LLS) - Nano Spring (NS - 30% caprolactone + 70% polychloric acid, biodegradation time 365 days) - these are springs, fastened on the needle-conductor, which also have the ability to swell) - Light Lift Series (Light Lift Spring – LLS –long thread-spring,) made from caprolactone, which is a biosynthetic material. The resorption of the thread occurs during the year, and the effect of its use persists up to 2 years. A separate control group was made up of 10 rats, which carried out impaltation of the same suture material, followed by its removal immediately after manipulation. Used mainly in patients under the age of 40 years who have an initial stage of ovate of the face. The specified threads have in their composition the L-isomer of lactic acid, which has revitalizing properties and slows down the aging process. All animals were kept in the vivarium of Danylo Halytsky Lviv National Medical University; experiments were conducted in accordance with the provisions of the European Convention for the protection of vertebrate animals used for experimental and other scientific purposes (Strasbourg, 1986), Council Directive 86/609/EEC (1986), Law of Ukraine No. 3445-IV "On the Protection of Animals from Cruelty". Animals were removed from the experiment by overdose of etheric anesthesia (5-7 min. exposure) on the 14th, 30th, 90th, 180th, 365th and 540th day of the experiment. Fragments of skin with subcutaneous fatty tissue from the back area [1] were used for the morphological study. Paraffin blocks were made from each fragment; histologic staining by routine method and Malory histochemical staining were performed. Results. During study of morphological changes in the perimplant zone, it was found that in all groups of exper-imental rats, on different chronological stages the collagen with different biodegradation time and in different quantities is formed. Dependence between type of implantation thread (structure) and stimulation of collagenogenesis has been established. Conclusions: 1. While studying morphological changes in the perimplant zone, it was found that in all groups of experimental rats, collagen with different term of biodegradation and in different quantities on various chrono-logical stages is formed. 2.It was established that collagenogenesis is stimu-lated unevenly, depending on the type of implantation thread (chemical composition and structure). 3.On the 365th day of the experiment, the resorbing thread did not completely degrade, which does not contradict the information provided by the manufacturer about the period of disintegration (365 days), and the expediency of using these cosmetic threads in aesthetic dermatology for the purpose of prolonged lifting skin correction. 4.The presence of polylactic acid in the filament is not an indicator of the effectiveness of collagenogenesis. To a greater extent, obviously, the actual structure of the ligature influences itself. 5.Immunohistochemical staining showed that in both groups of subjects collagen type 1 was formed in the perimplant area, , indicating a long-term effect after implan-tation.