THE ONTOGENETIC PECULIARITIES OF EFFECTS OF XENOGENIC CEREBROSPINAL FLUID ON RATS' LUNGS AT PARENTERAL ADMINISTRATION

Introduction and purpose. The cerebrospinal fluid contains many biologically active substances, which have a broad influence on different points of application in the organism. We performed the research of the influence of cerebrospinal fluid on the rats’ lungs (in the connection with the studies, provided in our department and devoted to influence of xenogenic cerebrospinal fluid (XCSF) on the organism of rats). Materials and methods. The investigation was performed on 84 Wistar rats of both sexes, which were divided into the experimental and control groups. The experiment was performed on impuberal (group 1), puberal (group 2) and aged rats (group 3). The duration of the experiment was 7 (for the groups 1 and 2), 30 (for all groups) and 90 days (for the groups 2 and 3). The rats of the experimental group got XCSF at the dose 0.002 ml/g three times with the interval of 2 days (for 7-day experiment) or ten times (for 30- and 90-day experiments). The control group received 0.9 % NaCl using the same dosage schedule. After decapitation (made under the general anesthesia) the lungs of the rats were fixed in 10 % formaldehyde. The histological specimens were stained with hematoxylin-eosin and with van Gieson's stain. The microscopy was performed using the light microscope Olympus CX-41, the morphometry was performed using the program ?DP Soft?. During the investigation we counted the relative amount of the areas with normal parenchyma, emphysema, dystelectasises, haemorrhages; we also studied the collagen fibers. Discussion. The triple introduction of XCSF to the animals of the group 1 caused growth of the relative amount of normal parenchyma by 14.35 % (р < 0.001) at the expense of decrease of relative amount of emphysematous areas by 15.95 % (р < 0.001). For group 2 the triple introduction caused less evident changes in comparison with the group 1: emphysematous areas decreased by 13.73 % (р < 0.001) at the expense of increase of the relative amount of the areas with normal parenchyma by 5.07 % (р < 0.01) and dystelectatic areas by 9.23 % (р < 0.001). During the 30-day experiment at the rats of the groups 2 and 3 the areas of normal parenchyma decreased by 8.85 % (р < 0.001) and 12.22 % (р < 0.001) respectively, while the amount of the emphysematous areas increased by 10.45 % (р < 0.001) in the group 2 and by 9.71 (р < 0.001) in the group 3. On 90th day we pointed out the growth of the relative amount of the normal parenchyma by 20.93 % (р < 0.001) due to the decrease of the relative amount of the emphysematous areas by 9.35 % (р < 0.001) and of the dystelectatic areas by 11.59 % (р < 0.001) in the group 2. In the group 3 the same, but less evident changes were found: the amount of the normal parenchyma increased by 6.85 % (р < 0.01), while emphysematous areas decreased by 5.56 % (р < 0.05) and dystelectatic areas decreased by 1.23 % (р < 0.01). Under the picrofuchsin staining we noticed the decrease of collagen’s content at the animals, who had received XCSF.