MORPHOLOGICAL CHARACTERISTICS OF REPARATIVE OSTEOGENESIS WITH THE USE OF CORAL-BLOOD MIXTURE IN THE EXPERIMENT

Aim. To study the morphological features of reparative osteogenesis when using granules of the skeleton of a coral of the Acroporidae family of the genus Acropora during its incubation with autologous blood. Materials and Methods. In an experiment on laboratory animals – Wistar rats, the osteoinductive properties of the granules of the natural coral skeleton Acroporidae were studied by mixing it with the blood of the animal and incubating it for 12 hours at a temperature of 4°C. The prepared mixture was filled with a defect in the femur. Results. It was found that with the use of this mixture, the processes of reparative osteogenesis are more effective than using mixtures of granules of coral and hydroxyapatite mixed with the blood of the animal just before surgery. On the 14th day, using our technique, in the fields of vision, pronounced regenerative angiogenesis and a significant proportion of fibrocytes in the macrophage-fibroblasto-endothelial histone were recorded. On the 21st day there was a proliferation of reticulofibrous bone tissue, surrounded by polymorphic bone marrow cells. During this period, the stabilization of the vascular fraction and the increase in the number of osteocytes in the regenerate were noted. By the 28th day, formation of lamellar bone tissue with Havers canals was revealed, marked ossification of the regenerate. The study showed that the coral has osteoinductive and osteoconductive properties. This confirms the formation of bone structures both when using the incubated mixture, and when directly mixed with the blood of the experimental animal before the operation. At the same time, the processes of bone tissue proliferation and defect regeneration are more pronounced when applying the proposed method. Conclusions. A method of the preparation of osteoplastic material to close the critical defects of the tubular bones on the basis of natural skeleton granules of the coral of the genus Acropora was developed and tested. Incubated material is more effective at closing the bone defect due to its plasticity. The proposed method simplifies the preparation of the filling mass, and also accelerates the healing process of bone wounds.