FORMULASI DAN EVALUASI MIKROEMULGEL DARI EKSTRAK DAUN SIRSAK (Annona muricata L.)

Staphylococcus aureus is one of the bacteria that can cause acne. One of the potential crop as antiacne is soursop leaves. This research was conducted to develop and evaluation of formulation soursop leaf extract that has good stability. This research begins by extracting the leaves of the soursop through maceration, fermentation then comparing the minimum inhibitory concentration which one is more effective as an antibacterial against Staphyloccoccus aureus. Afterwards, 9 microemulsion formula with variations in the concentration and type of cosurfactant used Tegocare® and Plantacare® were prepared. Selection of the best microemulsion formula decided by organoleptic evaluation. Microemulsion can be characterized by freeze and thaw, centrifugation and TEM (Transmission Electron Microscopy) tests. Evaluation on microemulgel preparations include organoleptic, pH, viscosity, homogeneity, panelists study and the scattering ability during 28 days of storage. The results showed that there was no significant difference (sig > 0.05) between fermented and nonfermented extract, so the chosen non-fermented extract into a formulation with a concentration of 10 mg/ml. Microemulsion with 10% of Plantacare® cosurfactant was microemulsion formula selected. In freeze and thaw tests, it was proofed that microemulsions were stable until the fifth cycle, while the TEM on microemulsion had a particle size of 50.0 nm. Selected microemulsion was incorporated into gel base formula 3 (Viscolam®) with various concentrations of 8% - 10%. The results of the analysis using ANOVA, the pH value in the third formula was not constant (sig < 0.05), while for viscosity values formula II (concentration 9%) was constant (sig > 0.05).