The Content of Prostaglandin F2alpha in Menstrual Blood of Women of Reproductive Age

The article provides information on the possibility of using PG F2α as a marker of the menstrual blood. Material and methods. We studied dried on gauze menstrual blood, taken from practically healthy women and women who had inflammatory diseases of the external genital organs, aged from 18 to 45 with a normal menstrual cycle. This method is new in forensic practice. Results and discussion. The obtained results allowed us to recommend this method for the forensic medical determination of the regional origin of blood. Menstruation (from Latin mensis - month), lunar or regulated is a part of the menstrual cycle of the female body during which there is a rejection of the functional layer of the endometrium (uterine mucosa), which is accompanied by bleeding. The main role in the beginning of menstruation is spasm of arterioles. It is known that vasoconstrictors, which are PG F2a, endothelial-1 and platelet activating factor (TAF), are produced within the limits of the endometrium and are involved in the reduction of blood vessels. They also promote the onset of menstruation and further control of it. These mediators are regulated by the effects of vasodilators such as PG E2, prostacyclin, nitric oxide, which are also produced by the endometrium. PG F2a has a pronounced vasoconstrictor effect, enhances spasm of arteries and endometrial ischemia, causes reduction of myometrium, which, on the one hand, reduces blood flow, on the other hand, promotes the removal of endometrium rejection. Menstrual blood is not coagulated and has a darker color than blood circulating in vessels. This is due to a number of enzymes existing in menstrual blood. We also studied the possibility of using PG F2α as a marker of menstrual blood origin. We analyzed the correlation of the content of PG F2α in menstrual blood, taking into account the presence of inflammatory diseases of the external genitalia. Thus, at the baseline level of PG F2α during menstruation, its content in menstrual blood was 14 ± 0.07 ng/mg. That is, during menstruation, the content of PGF2α is determined as a synthesis in the vaginal fluid, and in the uterine envelope. As for inflammatory diseases of the external genital organs, the base content of PG F2α in menstruation blood was 16.24 ± 0.04 ng/mg, which is 35.5 ng/mg higher. Thus, the synthesis of PGF2α in the vaginal fluid increased in the inflammatory processes of the external genital organs, because the uterine mucus was not involved in the inflammatory process. Conclusions. The conducted study showed that PG F2α was a characteristic constant component of menstrual blood. This is due to its participation in the physiological process of the onset of menstruation. Its blood content, which is 13.1 ng/mg or more, proves the menstrual origin of blood.